/
/
Production of Human Neurogenin 2-Inducible Neurons in a Three-Dimensional Suspension Bioreactor
This content is Free Access.
Production of Human Neurogenin 2-Inducible Neurons in a Three-Dimensional Suspension Bioreactor
1,076 Views
•
07:21 min
•
March 17, 2023
DOI:
1Fraunhofer Project Center for Stem Cell Process Engineering,Fraunhofer Institute for Biomedical Engineering IBMT, 2Bioneer A/S, 3Fraunhofer UK Research Ltd, Technology and Innovation Centre, 4Fraunhofer Institute for Biomedical Engineering IBMT, 5Department of Molecular and Cellular Biotechnology,Saarland University, 6Facultad de Ciencias del Mar,Universidad Católica del Norte
Summary
Automatically generated
This article describes a protocol for the generation of human induced pluripotent stem cell-derived neurons in a benchtop 3D suspension bioreactor.
Explore More Videos
3D Suspension BioreactorHuman Neurogenin 2-inducible NeuronsInduced Pluripotent Stem CellsIPSC CultureNeuronal DifferentiationHigh-throughput ScreeningRapid And Cost-efficient GenerationCardiomyocytesPre-cultivationTrypsinizationCell SingularizationCell CountingCell SuspensionCentrifugationFeeder-free IPSC Maintenance MediumROCK InhibitorBioreactor Cultivation
Read Article
Cite this Article
Wihan, J., Karnatz, I., Sébastien, I., Kettenhofen, R., Schmid, B., Clausen, C., Fischer, B., Steeg, R., Zimmermann, H., Neubauer, J. C. Production of Human Neurogenin 2-Inducible Neurons in a Three-Dimensional Suspension Bioreactor. J. Vis. Exp. (193), e65085, doi:10.3791/65085 (2023).
Copy