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Automatisierte multimodale Stimulation und simultane neuronale Ableitung von mehreren kleinen Organismen
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Neurociência
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JoVE Journal Neurociência
Automated Multimodal Stimulation and Simultaneous Neuronal Recording from Multiple Small Organisms
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Automatisierte multimodale Stimulation und simultane neuronale Ableitung von mehreren kleinen Organismen

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08:28 min

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March 03, 2023

DOI:

10.3791/65042-v

DOI:
10.3791/65042-v

•

08:28 min
March 03, 2023

•

9 Views
, , , ,
1Department of Biomedical Engineering,Worcester Polytechnic Institute, 2Graduate School of Biomedical Sciences,UMass Chan Medical School, 3Department of Biology and Biotechnology,Worcester Polytechnic Institute

Summary

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Wir stellen eine Methode zur flexiblen chemischen und multimodalen Stimulation und Aufzeichnung simultaner neuronaler Aktivität von vielen Caenorhabditis elegans-Würmern vor. Diese Methode nutzt Mikrofluidik, Open-Source-Hard- und Software sowie überwachte automatisierte Datenanalyse, um die Messung neuronaler Phänomene wie Anpassung, zeitliche Hemmung und Reizübersprechen zu ermöglichen.

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Cite this Article

White, H., Kamara, V., Gorski, V., Busby, M., Albrecht, D. R. Automated Multimodal Stimulation and Simultaneous Neuronal Recording from Multiple Small Organisms. J. Vis. Exp. (193), e65042, doi:10.3791/65042 (2023).

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