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Automated Multimodal Stimulation and Simultaneous Neuronal Recording from Multiple Small Organisms
Chapters
- 00:04Introduction
- 00:48Microfluidic Device Preparation
- 02:04Animal Loading
- 03:02Automated Stimulation and Neuronal Recording
- 04:15Data Analysis Using NeuroTracker
- 06:08Data Exploration and Visualization
- 06:47Results: Assessment of Temporal Inhibition, Disinhibition, and Multimodal Stimulation
- 07:51Conclusion
We present a method for the flexible chemical and multimodal stimulation and recording of simultaneous neural activity from many Caenorhabditis elegans worms. This method uses microfluidics, open-source hardware and software, and supervised automated data analysis to enable the measurement of neuronal phenomena such as adaptation, temporal inhibition, and stimulus crosstalk.
