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下丘脑Kisspeptin神经元作为全细胞膜片钳记录的靶标
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Neurociência
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JoVE Journal Neurociência
Hypothalamic Kisspeptin Neurons as a Target for Whole-Cell Patch-Clamp Recordings
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下丘脑Kisspeptin神经元作为全细胞膜片钳记录的靶标

DOI:
10.3791/64989-v

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09:39 min

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March 17, 2023

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, , ,
1Department of Anatomy, Instituto de Ciencias Biomedicas,Universidade de Sao Paulo

Capítulos

  • 00:05Introduction
  • 00:48Brain Dissection and Slicing
  • 02:49Cell Sealing for Patch‐Clamp Recording
  • 07:29Results: Whole‐Cell Patch‐Clamp Recordings on the Brain Slices
  • 08:39Conclusion

Summary

Tadução automática

Tadução automática

在这里，我们提出了一种协议，用于对含有kisspeptin神经元的脑切片进行全细胞膜片钳，kisspeptin神经元是促性腺激素释放激素（GnRH）细胞的主要调节剂。通过增加有关kisspeptin神经元活性的知识，这种电生理工具已成为过去20年来神经内分泌领域取得重大进展的基础。

Tags

Keywords: Whole-cell Patch-clamp RecordingsElectrophysiological PropertiesCell MembranesIonic CurrentsCellular ResponseBrain DissectionHypothalamusOptic NerveVibratomeACSF SolutionMicroscopeAmplifierDigitizerMicromanipulatorPerfusion Pump

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