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Hypothalamic Kisspeptin Neurons as a Target for Whole-Cell Patch-Clamp Recordings
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Hypothalamic Kisspeptin Neurons as a Target for Whole-Cell Patch-Clamp Recordings
Capitoli
- 00:05Introduction
- 00:48Brain Dissection and Slicing
- 02:49Cell Sealing for Patch‐Clamp Recording
- 07:29Results: Whole‐Cell Patch‐Clamp Recordings on the Brain Slices
- 08:39Conclusion
Here, we present a protocol to perform a whole-cell patch-clamp on brain slices containing kisspeptin neurons, the primary modulator of gonadotrophin-releasing hormone (GnRH) cells. By adding knowledge about kisspeptin neuron activity, this electrophysiological tool has served as the basis for significant advancements in the neuroendocrinology field over the last 20 years.
