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Hypothalamic Kisspeptin Neurons as a Target for Whole-Cell Patch-Clamp Recordings
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Hypothalamic Kisspeptin Neurons as a Target for Whole-Cell Patch-Clamp Recordings
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09:39 min
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March 17, 2023
DOI:
Summary
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Here, we present a protocol to perform a whole-cell patch-clamp on brain slices containing kisspeptin neurons, the primary modulator of gonadotrophin-releasing hormone (GnRH) cells. By adding knowledge about kisspeptin neuron activity, this electrophysiological tool has served as the basis for significant advancements in the neuroendocrinology field over the last 20 years.
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Silva, J. d. N., Zampieri, T. T., Vieira, H. R., Frazao, R. Hypothalamic Kisspeptin Neurons as a Target for Whole-Cell Patch-Clamp Recordings. J. Vis. Exp. (193), e64989, doi:10.3791/64989 (2023).
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