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"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome
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JoVE Journal Bioquímica
“Cell Surface Capture” Workflow for Label-Free Quantification of the Cell Surface Proteome
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"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome

DOI:
10.3791/64952-v

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06:31 min

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March 24, 2023

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, ,
1Department of Laboratory Medicine,University of California, San Francisco, 2Department of Biosciences and Bioengineering,Indian Institute of Technology Bombay, 3Deptartment of Bioengineering and Therapeutic Sciences,University of California, San Francisco, 4Chan Zuckerberg Biohub

Summary

Tadução automática

Tadução automática

Here, we describe a proteomics workflow for characterization of the cell surface proteome of various cell types. This workflow includes cell surface protein enrichment, subsequent sample preparation, analysis using an LC-MS/MS platform, and data processing with specialized software.

Tags

Cell Surface ProteomeCell Surface CaptureBiotin LabelingNeutrAvidin EnrichmentMass SpectrometryCancer ImmunotherapyCell Surface Antigens

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