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Ett-trinns CRISPR-basert strategi for endogen genmerking i Drosophila melanogaster

JoVE Journal
Neurociência

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JoVE Journal Neurociência

One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster

Ett-trinns CRISPR-basert strategi for endogen genmerking i Drosophila melanogaster

334 Views

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07:23 min

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January 26, 2024

DOI:

10.3791/64729-v

DOI:

10.3791/64729-v

•

07:23 min

January 26, 2024

•

19 Views

Yangbo Xiao*¹, Ye Yuan*², Swathi Yadlapalli^2,3

¹Department of Cell and Developmental Biology,University of Michigan, ²Cellular and Molecular Biology Program,University of Michigan, ³Michigan Neuroscience Institute Affiliate,University of Michigan

Tags

CRISPR Gene Tagging Drosophila Melanogaster Homologous Recombination Fluorescent Protein Knock-in SgRNA Donor Vector Cloning Plasmid Sanger Sequencing

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