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Ett-trinns CRISPR-basert strategi for endogen genmerking i Drosophila melanogaster
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Neurociência
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JoVE Journal Neurociência
One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster

Ett-trinns CRISPR-basert strategi for endogen genmerking i Drosophila melanogaster

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07:23 min

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January 26, 2024

DOI:

10.3791/64729-v

DOI:
10.3791/64729-v

•

07:23 min
January 26, 2024

•

5 Views
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1Department of Cell and Developmental Biology,University of Michigan, 2Cellular and Molecular Biology Program,University of Michigan, 3Michigan Neuroscience Institute Affiliate,University of Michigan

Summary

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Her presenterer vi en forenklet endogen genmerkingsprotokoll for Drosophila, som benytter en PCR-basert teknikk for markørfri identifisering av vellykkede genetiske modifikasjoner, noe som letter utviklingen av stabile innslagslinjer.

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Cite this Article

Xiao, Y., Yuan, Y., Yadlapalli, S. One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster. J. Vis. Exp. (203), e64729, doi:10.3791/64729 (2024).

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