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Generazione di organoidi dei vasi sanguigni umani da cellule staminali pluripotenti
JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia
Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells
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Generazione di organoidi dei vasi sanguigni umani da cellule staminali pluripotenti

4,293 Views

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09:46 min

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January 20, 2023

DOI:

10.3791/64715-v

DOI:
10.3791/64715-v

•

09:46 min
January 20, 2023

•

54 Views
,
1School of Biomedical Engineering,University of British Columbia, 2Life Sciences Institute,University of British Columbia, 3Department of Medical Genetics,University of British Columbia, 4Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)

Summary

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Questo protocollo descrive la generazione di vasi sanguigni auto-organizzanti da cellule staminali pluripotenti e pluripotenti indotte umane. Queste reti di vasi sanguigni mostrano una rete endoteliale estesa e connessa circondata da periciti, actina muscolare liscia e una membrana basale continua.

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Cite this Article

Werschler, N., Penninger, J. Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells. J. Vis. Exp. (191), e64715, doi:10.3791/64715 (2023).

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