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使用扩增显微镜物理放大 整个果蝇 胚胎以进行超分辨率成像

JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento

Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging

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使用扩增显微镜物理放大整个果蝇胚胎以进行超分辨率成像

DOI:

10.3791/64662-v

•

09:11 min

•

April 28, 2023

•

Samia Parveen, Nicolas W. Jones, Ian Millerschultz, Adam C. Paré

¹Department of Biological Sciences,University of Arkansas

Capítulos

00:05Introduction
00:53Drosophila Embryo Fixation and Devitellinization
03:04Preparing PDMS Wells
03:57Adhering the Embryos to the Coverslips
04:33Activation and Gelation
05:48Digestion and Expansion
06:33Mounting and Imaging
07:08Results: Analysis of Unexpanded and Expanded Embryos
08:45Conclusion

Summary

Tadução automática

在这里，提出了在早期果蝇胚胎中实施扩展显微镜以实现使用常规激光扫描共聚焦显微镜实现超分辨率成像的方案。

Tags

Keywords: Expansion Microscopy Whole-mount Drosophila Embryos Super-resolution Imaging Protein Localization Cell Morphology Agar Slab Double-sided Tape Vitelline Membrane Poly-L-Lysine PBS Tween

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