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从健康和视网膜疾病特异性人诱导的多能干细胞中生成视网膜类器官
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December 09, 2022
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该协议描述了一种将hiPSCs分化为眼场簇并使用涉及贴壁和悬浮培养系统的简化培养条件生成神经视网膜类器官的有效方法。其他眼细胞类型，如RPE和角膜上皮，也可以在视网膜培养物中从成熟的眼视野中分离出来。
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Mahato, S., Agrawal, T., Pidishetty, D., Maddileti, S., Pulimamidi, V. K., Mariappan, I. Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells. J. Vis. Exp. (190), e64509, doi:10.3791/64509 (2022).
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