/
/
Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells
2,785 Views
•
09:47 min
•
December 09, 2022
DOI:
Summary
Automatically generated
This protocol describes an efficient method of differentiating hiPSCs into eye field clusters and generating neuro-retinal organoids using simplified culture conditions involving both adherent and suspension culture systems. Other ocular cell types, such as the RPE and corneal epithelium, can also be isolated from mature eye fields in retinal cultures.
Explore More Videos
Retinal OrganoidsHuman Induced Pluripotent Stem CellsRetinal DegenerationRetinal Pigment EpitheliumIn Vitro ModelsCell-based TherapiesEye DevelopmentInherited Retinal DiseasesDifferentiationAdherent CultureSuspension CultureRetinal CupsSpatial PositioningMorphological FeaturesCell DissociationIPSC CultureDifferentiation Induction MediumBFGFNoggin
Read Article
Cite this Article
Mahato, S., Agrawal, T., Pidishetty, D., Maddileti, S., Pulimamidi, V. K., Mariappan, I. Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells. J. Vis. Exp. (190), e64509, doi:10.3791/64509 (2022).
Copy