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Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells
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December 09, 2022
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This protocol describes an efficient method of differentiating hiPSCs into eye field clusters and generating neuro-retinal organoids using simplified culture conditions involving both adherent and suspension culture systems. Other ocular cell types, such as the RPE and corneal epithelium, can also be isolated from mature eye fields in retinal cultures.
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Retinal OrganoidsHuman Induced Pluripotent Stem CellsRetinal DegenerationRetinal Pigment EpitheliumIn Vitro ModelsCell-based TherapiesEye DevelopmentInherited Retinal DiseasesDifferentiationAdherent CultureSuspension CultureRetinal CupsSpatial PositioningMorphological FeaturesCell DissociationIPSC CultureDifferentiation Induction MediumBFGFNoggin
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Mahato, S., Agrawal, T., Pidishetty, D., Maddileti, S., Pulimamidi, V. K., Mariappan, I. Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells. J. Vis. Exp. (190), e64509, doi:10.3791/64509 (2022).
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