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Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins
 

Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins

Article DOI: 10.3791/64473-v • 10:21 min • January 13th, 2023
January 13th, 2023 •
1, 1, 1, 1
1Department of Pharmacy, University of Oslo

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A protocol is presented for the determination of low-abundance biomarkers from dried serum samples exemplified with the biomarker progastrin-releasing peptide (ProGRP). Antibody-coated magnetic beads are used for the selective cleanup and enrichment of a proteotypic ProGRP peptide. The captured peptide is subsequently analyzed by liquid chromatography-tandem mass spectrometry.

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Keywords: Microsampling Targeted Mass Spectrometry Protein Analysis Low-abundance Proteins Affinity Cleanup Protein Proteolysis Antibody PH Adjustment Magnetic Beads Antibody Coupling Borate Buffer Coupling Buffer
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