/

/

在2D结构中模拟人类小脑体外发育

JoVE Journal
Neurociência

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Neurociência

Modeling Human Cerebellar Development In Vitro in 2D Structure

Please note that all translations are automatically generated. Click here for the English version.

在2D结构中模拟人类小脑体外发育

DOI:

10.3791/64462-v

•

06:14 min

•

September 16, 2022

•

Deniz A. Madencioglu^3,4,5, Karina A. Kruth^3,4,5, Thomas H. Wassink³, Vincent A. Magnotta^2,3,4,5, John A. Wemmie^3,4,5, Aislinn J. Williams^3,4,5

¹Department of Psychiatry,University of Iowa, ²Department of Radiology,University of Iowa, ³Carver College of Medicine,University of Iowa, ⁴Iowa Neuroscience Institute,University of Iowa, ⁵Pappajohn Biomedical Institute,University of Iowa

Capítulos

00:04Introduction
00:41Experimental Preparation
01:15Cerebellar Differentiation
03:59Results: Overview of the 3D to 2D Cerebellar Differentiation and Expression of Cerebellar Cell Markers
05:48Conclusion

Summary

Tadução automática

本协议解释了从诱导多能干细胞生成2D单层小脑细胞，用于研究小脑发育的早期阶段。

Tags

Keywords: 2D Structure In Vitro Cerebellar Development Human Neuropsychiatric Disorders Cost-efficient Pulled Glass Pipette IPSC Embryoid Bodies Cerebellar Differentiation Medium FGF2

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

在鼠标的操作性感觉寻求

在发展中国家斑马鱼的前脑的神经祖细胞克隆相关的定时实时成像

使用扩频萧条的情节和慢性偏头痛的神经免疫信号建模<em在体外</em

小脑颗粒神经元的遗传操作<em>体外</em>和<em>在体内</em>为研究神经元形态和迁移

小脑轴突的激光Nanosurgery<em>在体内</em

建模星形细胞瘤发病机制<em>在体外</em>和<em>体内</em>使用星形胶质细胞或条件，遗传工程小鼠神经干细胞

在癌神经侵袭的体外模型：背根神经节型号

小鼠小脑颗粒神经元神经元死亡和变性的模型研究

小鼠原代多元体的体外模拟？

成人脑切片长期抑郁症诱导评估

Read Article