Journal
/
神经科学
/
Modeling Human Cerebellar Development In Vitro in 2D Structure
JoVE 杂志
神经科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
Modeling Human Cerebellar Development In Vitro in 2D Structure

Modeling Human Cerebellar Development In Vitro in 2D Structure

DOI:
10.3791/64462-v

•

06:14 min

•

September 16, 2022

•

, , , , ,
1Department of Psychiatry,University of Iowa, 2Department of Radiology,University of Iowa, 3Carver College of Medicine,University of Iowa, 4Iowa Neuroscience Institute,University of Iowa, 5Pappajohn Biomedical Institute,University of Iowa

Chapters

  • 00:04Introduction
  • 00:41Experimental Preparation
  • 01:15Cerebellar Differentiation
  • 03:59Results: Overview of the 3D to 2D Cerebellar Differentiation and Expression of Cerebellar Cell Markers
  • 05:48Conclusion

Summary

自动翻译

自动翻译

The present protocol explains the generation of a 2D monolayer of cerebellar cells from induced pluripotent stem cells for investigating the early stages of cerebellar development.

Tags

2D StructureIn VitroCerebellar DevelopmentHumanNeuropsychiatric DisordersCost-efficientPulled Glass PipetteIPSCEmbryoid BodiesCerebellar Differentiation MediumFGF2

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord

Operant Sensation Seeking in the Mouse

Time-lapse Live Imaging of Clonally Related Neural Progenitor Cells in the Developing Zebrafish Forebrain

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

Laser Nanosurgery of Cerebellar Axons In Vivo

Modeling Astrocytoma Pathogenesis In Vitro and In Vivo Using Cortical Astrocytes or Neural Stem Cells from Conditional, Genetically Engineered Mice

In Vitro Modeling of Cancerous Neural Invasion: The Dorsal Root Ganglion Model

Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons

Modeling Charcot-Marie-Tooth Disease In Vitro by Transfecting Mouse Primary Motoneurons

Read Article