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Imunologia e Infecção
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Use of In Vivo Imaging to Screen for Morphogenesis Phenotypes in Candida albicans Mutant Strains During Active Infection in a Mammalian Host
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Use of In Vivo Imaging to Screen for Morphogenesis Phenotypes in Candida albicans Mutant Strains During Active Infection in a Mammalian Host

Use of In Vivo Imaging to Screen for Morphogenesis Phenotypes in Candida albicans Mutant Strains During Active Infection in a Mammalian Host

DOI:
10.3791/64258-v

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09:24 min

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October 12, 2022

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, ,
1Department of Pediatrics, Carver College of Medicine,University of Iowa, 2Department of Microbiology and Immunology, Carver College of Medicine,University of Iowa

Capítulos

  • 00:04Introduction
  • 00:52Candida albicans Strains and Animal Preparation
  • 02:08Hair Removal and Inoculation
  • 04:06Quantify In Vitro Morphogenesis for Comparison to In Vivo Results
  • 04:42Preparation for In Vivo Imaging
  • 06:37Imaging
  • 07:38Manual Two‐Dimensional Analysis: Frequency of Filamentation
  • 07:59Results: Analyzing In Vitro and In Vivo Morphology of the Mutant Strains
  • 08:51Conclusion

Summary

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This manuscript describes a method for screening moderately sized Candida albicans mutant libraries for morphogenesis phenotypes during active infection in a mammalian host using non-invasive confocal microscopy.

Tags

In Vivo ImagingCandida AlbicansMorphogenesisMammalian HostIntradermal InjectionChlorophyll-free ChowYPDDPBSHemocytometerDepilatory Cream70% EthanolInsulin Syringe

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