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Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics
JoVE Journal
Biologia
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JoVE Journal Biologia
Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics

Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics

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09:10 min

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August 25, 2022

DOI:

10.3791/64228-v

DOI:
10.3791/64228-v

•

09:10 min
August 25, 2022

•

39 Views
, , , , , , , ,
1Department of Physics and Biophysics,University of San Diego, 2W. M. Keck Science Department,Scripps College, Pitzer College, and Claremont McKenna College, 3Department of Physics,Syracuse University

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This paper presents protocols for engineering and characterizing tunable three-dimensional composite networks of co-entangled actin filaments and microtubules. Composites undergo active restructuring and ballistic motion, driven by myosin II and kinesin motors, and are tuned by the relative concentrations of actin, microtubules, motor proteins, and passive crosslinkers.

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Sasanpour, M., Achiriloaie, D. H., Lee, G., Leech, G., Hendija, M., Lindsay, K. A., Ross, J. L., McGorty, R. J., Robertson-Anderson, R. M. Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics. J. Vis. Exp. (186), e64228, doi:10.3791/64228 (2022).

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