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Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics

Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics

DOI:
10.3791/64228-v

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09:10 min

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August 25, 2022

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, , , , , , , ,
1Department of Physics and Biophysics,University of San Diego, 2W. M. Keck Science Department,Scripps College, Pitzer College, and Claremont McKenna College, 3Department of Physics,Syracuse University

Chapters

  • 00:05Introduction
  • 01:01Preparation of Active Actin-Microtubule Composites Driven by Kinesin Motors
  • 03:39Incorporating Passive Crosslinkers into Active Composites
  • 05:11Results: Engineering and Characterizing the Tunable Dynamics and Structure of Motor-Driven Actin-Microtubule Composites
  • 08:20Conclusion

Summary

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This paper presents protocols for engineering and characterizing tunable three-dimensional composite networks of co-entangled actin filaments and microtubules. Composites undergo active restructuring and ballistic motion, driven by myosin II and kinesin motors, and are tuned by the relative concentrations of actin, microtubules, motor proteins, and passive crosslinkers.

Tags

ActinMicrotubulesCytoskeletonReconstitutionCompositesMotor-driven DynamicsMechanicsKinesinMyosinActive MatterContractionExtensionCoarseningRuptureMicroscopyConfocal Imaging

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