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快速有效地监测完整斑马鱼胚胎中正常和异常胞嘧啶甲基化的时空监测
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos
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快速有效地监测完整斑马鱼胚胎中正常和异常胞嘧啶甲基化的时空监测

DOI:
10.3791/64190-v

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07:16 min

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August 18, 2022

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,
1Department of Environmental Sciences,University of California, Riverside

Capítulos

  • 00:05Introduction
  • 01:02Zebrafish Embryo Collection and Chemical Exposure
  • 02:25Dechorionation of Embryos
  • 03:22Immunohistochemistry Using 5-mC-Specific Antibody
  • 04:46Automated Imaging of Embryos Within 96-Well Plates
  • 06:02Results: Optimization of the Custom Module for Detection of 5-mC
  • 06:42Conclusion

Summary

Tadução automática

Tadução automática

本文描述了一种快速有效地对完整斑马鱼胚胎内正常和异常胞嘧啶甲基化进行时空监测的方案。

Tags

Zebrafish Embryos5-MethylcytosineEpigenetic BiomarkerSpatiotemporal MonitoringCost-efficientRapidAutomated AcquisitionLow Sensitivity ScreeningChemical ExposureEnvironmental StimuliNCG MethodsParaformaldehyde FixationDechorionation

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