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JoVE Journal Neuroscience
Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain
 

Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain

Article DOI: 10.3791/63998-v • 09:44 min • May 20th, 2022
May 20th, 2022 •
1,2, 2, 3, 1,2
1Graduate School of Biomedical Science and Engineering, University of Maine, 2Department of Neurosurgery, Ohio State University Wexner Medical Center, 3Boston Children’s Hospital, Harvard Medical School

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Resumo
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The ability to permanently mark stem cells and their progeny with a fluorophore using an inducible transgenic lineage tracing mouse line allows for spatial and temporal analysis of activation, proliferation, migration, and/or differentiation in vivo. Lineage tracing can reveal novel information about lineage commitment, response to intervention(s), and multipotency.

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Lineage Tracing Inducible Fluorescently-labeled Stem Cells Adult Mouse Brain Transgenic Lineage Tracing Activation Analysis Proliferation Analysis Migration Analysis Differentiation Analysis Lineage Commitment Response To Interventions Multipotency Advantages Of Transgenic Lineage Tracing Fluorescent Protein Expression Doxycycline Toxicity Conditional Activation Downstream Assays Immunostaining Immunofluorescence Plasticity In The Adult Brain Neurodegenerative Diseases Metabolic Impacts On Brain Plasticity Aging Warm Slides Post-fixation With Acetone Rinse Buffer Permeabilization With Triton X-100 And Tween-20
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