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Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
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Biologia
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JoVE Journal Biologia
Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

4,124 Views

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08:55 min

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July 12, 2022

DOI:

10.3791/63923-v

DOI:
10.3791/63923-v

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08:55 min
July 12, 2022

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15 Views
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1Electron Bio-Imaging Centre,Diamond Light Source Ltd, Harwell Science & Innovation Campus, 2Division of Structural Biology, Wellcome Trust Centre for Human Genetics,University of Oxford, 3Chinese Academy of Medical Sciences Oxford Institute,University of Oxford

Summary

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The present protocol describes high-resolution cryo-electron tomography remote data acquisition using Tomo5 and subsequent data processing and subtomogram averaging using emClarity. Apoferritin is used as an example to illustrate detailed step-by-step processes to achieve a cryo-ET structure at 2.86 Å resolution.

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Sheng, Y., Morris, K., Radecke, J., Zhang, P. Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging. J. Vis. Exp. (185), e63923, doi:10.3791/63923 (2022).

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