Journal
/
Biologia
/
Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

4,078 Views

•

08:55 min

•

July 12, 2022

DOI:

10.3791/63923-v

DOI:
10.3791/63923-v

•

08:55 min
July 12, 2022

•

15 Views
, , ,
1Electron Bio-Imaging Centre,Diamond Light Source Ltd, Harwell Science & Innovation Campus, 2Division of Structural Biology, Wellcome Trust Centre for Human Genetics,University of Oxford, 3Chinese Academy of Medical Sciences Oxford Institute,University of Oxford

Summary

Automatically generated

The present protocol describes high-resolution cryo-electron tomography remote data acquisition using Tomo5 and subsequent data processing and subtomogram averaging using emClarity. Apoferritin is used as an example to illustrate detailed step-by-step processes to achieve a cryo-ET structure at 2.86 Å resolution.

Explore More Videos

Cryo-electron TomographyRemote Data CollectionSubtomogram AveragingCROWETMicromoleculesNative Cellular EnvironmentTomo Five SoftwareImage Acquisition ParametersAtlasScreeningTarget SetupImage Shift CalibrationEucentric Height AdjustmentLow Dose Tomography Setup

Read Article

Cite this Article

Sheng, Y., Morris, K., Radecke, J., Zhang, P. Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging. J. Vis. Exp. (185), e63923, doi:10.3791/63923 (2022).

Download .ris file

Copy

Share Video

.

Copy