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Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
JoVE Journal
Biology
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JoVE Journal Biology
Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

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08:55 min

July 12, 2022

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08:55 min
July 12, 2022

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Summary

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The present protocol describes high-resolution cryo-electron tomography remote data acquisition using Tomo5 and subsequent data processing and subtomogram averaging using emClarity. Apoferritin is used as an example to illustrate detailed step-by-step processes to achieve a cryo-ET structure at 2.86 Å resolution.

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