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Microfluïdica en fluorescentiemicroscopie gebruiken om de assemblagedynamiek van single actinefilamenten en -bundels te bestuderen
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Capítulos
- 00:04Introduction
- 00:40Polydimethylsiloxane (PDMS) Chamber Assembly
- 01:45Connect Microfluidic Device
- 03:11Configuring the Setup with Standard Flow Rates and Changing Solution
- 04:21Basic Single Filament Experiment: Adenosine Diphosphate (ADP)-Actin Barbed End Depolymerization
- 06:45Results: Polymerization of Actin Filaments from Spectrin-Actin Seeds when Exposed to Different Actin Binding Proteins
- 07:24Conclusion
We presenteren protocollen voor eenvoudige actinefilamentmicrofluïdische assays, in combinatie met fluorescentiemicroscopie, waarmee men individuele actinefilamenten in realtime nauwkeurig kan volgen terwijl ze sequentieel worden blootgesteld aan verschillende eiwitoplossingen.
