Journal
/
Biologia do Desenvolvimento
/
Generering af luftvejsepitelcelle luft-væske-grænsefladekulturer fra humane pluripotente stamceller
JoVE Journal
Biologia do Desenvolvimento
This content is Free Access.
JoVE Journal Biologia do Desenvolvimento
Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells

Generering af luftvejsepitelcelle luft-væske-grænsefladekulturer fra humane pluripotente stamceller

3,143 Views

•

10:46 min

•

June 14, 2022

DOI:

10.3791/63882-v

DOI:
10.3791/63882-v

•

10:46 min
June 14, 2022

•

8 Views
, , , , , , ,
1Pulmonary Center,Boston University School of Medicine, 2Center for Regenerative Medicine,Boston University and Boston Medical Center, 3Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine,University of Texas Health Science Center

Tags

Airway Epithelial CellsAir-liquid InterfaceHuman Pluripotent Stem Cells3D MatrixCell CultureCell DifferentiationBasal Cell MediumDispase IIDissociation

Read Article

Cite this Article

Berical, A., Beermann, M. L., Suzuki, S., LeSuer, J., Matte, T., Davis, B., Kotton, D., Hawkins, F. Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells. J. Vis. Exp. (184), e63882, doi:10.3791/63882 (2022).

Download .ris file

Copy

Share Video

.

Copy