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Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells
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発生生物学
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JoVE Journal 発生生物学
Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells

Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells

3,261 Views

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10:46 min

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June 14, 2022

DOI:

10.3791/63882-v

DOI:
10.3791/63882-v

•

10:46 min
June 14, 2022

•

17 Views
, , , , , , ,
1Pulmonary Center,Boston University School of Medicine, 2Center for Regenerative Medicine,Boston University and Boston Medical Center, 3Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine,University of Texas Health Science Center

概要

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Recent advances in human induced pluripotent stem cell differentiation protocols allow for the stepwise derivation of organ-specific cell types. Here, we provide detailed steps for the maintenance and expansion of iPSC-derived airway basal cells and their differentiation into a mucociliary epithelium in air-liquid interface cultures.

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Cite this Article

Berical, A., Beermann, M. L., Suzuki, S., LeSuer, J., Matte, T., Davis, B., Kotton, D., Hawkins, F. Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells. J. Vis. Exp. (184), e63882, doi:10.3791/63882 (2022).

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