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Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells
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Biología del desarrollo
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JoVE Revista Biología del desarrollo
Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells

Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells

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10:46 min

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June 14, 2022

DOI:

10.3791/63882-v

DOI:
10.3791/63882-v

•

10:46 min
June 14, 2022

•

8 Views
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1Pulmonary Center,Boston University School of Medicine, 2Center for Regenerative Medicine,Boston University and Boston Medical Center, 3Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine,University of Texas Health Science Center

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Airway Epithelial CellsAir-liquid InterfaceHuman Pluripotent Stem Cells3D MatrixCell CultureCell DifferentiationBasal Cell MediumDispase IIDissociation

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Berical, A., Beermann, M. L., Suzuki, S., LeSuer, J., Matte, T., Davis, B., Kotton, D., Hawkins, F. Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells. J. Vis. Exp. (184), e63882, doi:10.3791/63882 (2022).

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