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通过高分辨率呼吸测量法评估坐骨神经的线粒体功能
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JoVE Journal Bioquímica
Assessing Mitochondrial Function in Sciatic Nerve by High-Resolution Respirometry
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通过高分辨率呼吸测量法评估坐骨神经的线粒体功能

DOI:
10.3791/63690-v

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08:19 min

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May 05, 2022

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1Instituto de Bioquímica Médica Leopoldo de Meis, Centro de Ciências da Saúde,Universidade Federal do Rio de Janeiro

Capítulos

  • 00:04Introduction
  • 01:19Calibration of Polarographic Oxygen Sensors for High-Resolution Respirometry (HRR)
  • 01:56Dissection and Permeabilization of the Sciatic Nerve
  • 02:58Oxygen Consumption and Reactive Oxygen Species (ROS) Production Determination
  • 05:51Results: Assessing the Mitochondrial Oxygen Consumption by the Permeabilized Sciatic Nerve
  • 07:41Conclusion

Summary

Tadução automática

Tadução automática

高分辨率呼吸测量与荧光传感器相结合，可测定线粒体耗氧量和活性氧 （ROS） 生成。本方案描述了一种评估透化坐骨神经中线粒体呼吸频率和ROS产生的技术。

Tags

Mitochondrial FunctionSciatic NerveHigh-resolution RespirometryOxygen ConsumptionROS ProductionNeuropathiesDiabetesChemotherapyNeurobiologyBioquímicaPharmacologyMitochondrial Disease MechanismsMitochondrial BoostersNeuropathic PainPermeabilizationAmplex RedPeroxidase

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