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簡単なバイオアッセイによる海アネモンからの粗抽出物中の溶血性およびホスホリパーゼ活性の同定
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Identification of Hemolytic and Phospholipase Activity in Crude Extracts from Sea Anemones by Straightforward Bioassays
DOI:

12:12 min

March 29, 2022

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Capítulos

  • 00:04Introduction
  • 01:29Organism Collection and Tissue Hydration
  • 02:26Toxin Release
  • 03:21Quantitation of Total Protein
  • 04:03Determine Polypeptide Venom Complexity and Protein Staining
  • 05:24Prepare Red Blood Cell Solution
  • 06:31Hemolysis Assay
  • 07:24Phospholipase Assay
  • 08:46Results: Nematocyst Discharge, Electrophoretic Profile of Sea Anemone Crude Extract, Hemolysis Assay in Erythrocytes from Sheep, and Phospholipase Assay
  • 11:01Conclusion

Summary

Tadução automática

ここでは、イソギンチャクから粗毒抽出物を取得し、その溶血作用およびホスホリパーゼ活性を検出するためのプロトコールについて記載する。

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