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通过直接生物测定法鉴定海葵粗提取物中的溶血和磷脂酶活性
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Bioquímica
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JoVE Journal Bioquímica
Identification of Hemolytic and Phospholipase Activity in Crude Extracts from Sea Anemones by Straightforward Bioassays
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通过直接生物测定法鉴定海葵粗提取物中的溶血和磷脂酶活性

DOI:
10.3791/63630-v

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12:12 min

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March 29, 2022

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, , ,
1Instituto de Biotecnología,Universidad Nacional Autónoma de México, 2Departamento de Micro y Nanotecnologías,Instituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México

Capítulos

  • 00:04Introduction
  • 01:29Organism Collection and Tissue Hydration
  • 02:26Toxin Release
  • 03:21Quantitation of Total Protein
  • 04:03Determine Polypeptide Venom Complexity and Protein Staining
  • 05:24Prepare Red Blood Cell Solution
  • 06:31Hemolysis Assay
  • 07:24Phospholipase Assay
  • 08:46Results: Nematocyst Discharge, Electrophoretic Profile of Sea Anemone Crude Extract, Hemolysis Assay in Erythrocytes from Sheep, and Phospholipase Assay
  • 11:01Conclusion

Summary

Tadução automática

Tadução automática

在这里，我们描述了一种从海葵中获得粗毒血清提取物并检测其溶血和磷脂酶活性的方案。

Tags

Keyword Extraction:Hemolytic ActivityPhospholipase ActivitySea Anemone ToxinBioassaysCrude ExtractProteinHemolysisPhospholysisErythrocyteTemperatureLyophilizationCell LysisNematocyst DischargeCentrifugationBradford Colorimetric Assay

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