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Tension Gauge Tether Probes for Quantifying Growth Factor Mediated Integrin Mechanics and Adhesion
 

Tension Gauge Tether Probes for Quantifying Growth Factor Mediated Integrin Mechanics and Adhesion

Article DOI: 10.3791/63529-v • 09:56 min • February 11th, 2022
February 11th, 2022 •
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1Department of Cell, Developmental, and Integrative Biology, University of Alabama at Birmingham

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TGT surface is an innovative platform to study growth factor-integrin crosstalk. The flexible probe design, specificity of the adhesion ligand, and precise modulation of stimulation conditions allow robust quantitative assessments of EGFR-integrin interplay. The results highlight EGFR as a 'mechano-organizer' tuning integrin mechanics, influencing focal adhesion assembly and cell spreading.

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Tension Gauge Tether Probes Growth Factor Mediated Integrin Mechanics Adhesion TGT Sensor Platform EGFR-integrin Crosstalk Cell Mechanical Forces EGF-dependent Regulation Ligands Cell Types Stimulation Paradigms Tension Thresholds Surface Synthesis Reagent Preparation Calculations Glass Cover Slips Ethanol Sonicate Piranha Solution Etching Ultrapure Water
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