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测量果蝇光感受器中光色素水平的电生理学方法

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Electrophysiological Methods for Measuring Photopigment Levels in Drosophila Photoreceptors

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测量果蝇光感受器中光色素水平的电生理学方法

DOI:

10.3791/63514-v

•

08:09 min

•

June 02, 2022

•

Rita Gutorov, Ben Katz, Baruch Minke

¹Department of Medical Neurobiology, Faculty of Medicine and the Edmond and Lily Safra Center for Brain Sciences (ELSC),Hebrew University

Capítulos

00:04Introduction
00:50Measuring the Prolonged Depolarizing Afterpotential (PDA) and the Early Receptor Potential (ERP) Using the Electroretinogram (ERG)
05:28Results: Detecting Abnormalities in Photopigment Biogenesis by Measuring Photopigment Levels in Drosophila Using the Electroretinogram
07:13Conclusion

Summary

Tadução automática

我们提出了一种电生理学表征双稳定光色素的方案：（i）利用光子吸收后光色素分子内的电荷位移及其在光感受器中的大量电荷，以及（ii）利用视紫红质和偏视紫红质光色素状态的吸收光谱差异。这些方案可用于筛选影响双稳定光色素系统的突变。

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