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活斑马鱼幼虫的眼睛去除以检查视觉系统的神经支配依赖性生长和发育
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System
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活斑马鱼幼虫的眼睛去除以检查视觉系统的神经支配依赖性生长和发育

DOI:
10.3791/63509-v

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09:00 min

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February 11, 2022

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, , , ,
1Department of Biology,Reed College, 2Committee on Development, Regeneration, and Stem Cell Biology,University of Chicago, 3Department of Cell and Developmental Biology,University College London

Capítulos

  • 00:04Introduction
  • 00:49Electrolytically Sharpened Tungsten Needles
  • 01:45Preparing Larvae for Surgery
  • 03:08Eye-Removal and Postoperative Care
  • 04:27Dissecting Larvae to Reveal Brains
  • 06:03Mounting and Imaging
  • 07:01Results: Vertebrate Visual System Development in Zebrafish Larvae
  • 08:17Conclusion

Summary

Tadução automática

Tadução automática

本文解释了如何手术从活斑马鱼幼虫身上摘除眼睛，作为研究视网膜输入如何影响视神经结构生长和发育的第一步。此外，本文还提供了有关幼虫麻醉、固定和脑解剖的信息，然后是免疫组化和共聚焦成像。

Tags

Eye RemovalZebrafish LarvaeInnervation-dependent GrowthVisual SystemRetinal InputOptic TectumNeural DevelopmentRegenerationDegenerationSurgical TechniqueAnesthesiaImmobilizationAgarose

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