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1Centre for Women, Children, and Reproduction,Guangxi Key Laboratory of Metabolic Diseases Research, 2Department of Biopharmaceutics, School of Laboratory Medicine and Biotechnology,Southern Medical University, 3Department of Obstetrics and Gynecology,Chinese PLA General Hospital, 4Clinical Laboratory,Northern Theater Air Force Hospital, 5Guangzhou Darui Reproduction Technology Co., Ltd.
Capítulos
- 00:04Introduction
- 00:40Fragment Selection Procedure
- 02:20Sequencing Template Preparation and Enrichment
- 05:48Loading the Sample and Sequencing
- 07:22Results: Whole Genome Amplification Combined with Next-Generation Sequencing for Analysis of Embryo Aneuploidy
- 08:59Conclusion
Het protocol presenteert de algemene procedures in het laboratorium die vereist zijn bij pre-implantatie genetische tests voor aneuploïdie op een op halfgeleiders gebaseerd sequencingplatform van de volgende generatie. Hier presenteren we de gedetailleerde stappen van volledige genoomversterking, DNA-fragmentselectie, bibliotheekconstructie, sjabloonvoorbereiding en sequencing-werkstroom met representatieve resultaten.
