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Cuantificación de la distribución de glucógeno subcelular en fibras musculares esqueléticas mediante microscopía electrónica de transmisión
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Bioquímica
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JoVE Journal Bioquímica
Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy
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Cuantificación de la distribución de glucógeno subcelular en fibras musculares esqueléticas mediante microscopía electrónica de transmisión

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08:32 min

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February 07, 2022

DOI:

10.3791/63347-v

DOI:
10.3791/63347-v

•

08:32 min
February 07, 2022

•

2 Views
, , , ,
1Research center for applied health science,University College South Denmark, 2Department of Sports Science and Clinical Biomechanics,University of Southern Denmark, 3Department of Biomedical Sciences, Core Facility for Integrated Microscopy,University of Copenhagen

Tags

Glycogen DistributionSubcellularSkeletal Muscle FibersTransmission Electron MicroscopyHigh ResolutionAntibody-free StainingPotassium FerrocyanideGlutaraldehyde FixationOsmium TetroxideEpoxy Resin EmbeddingUltramicrotomySemi-thin SectioningLight Microscopy

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Jensen, R., Ørtenblad, N., di Benedetto, C., Qvortrup, K., Nielsen, J. Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy. J. Vis. Exp. (180), e63347, doi:10.3791/63347 (2022).

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