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संचरण इलेक्ट्रॉन माइक्रोस्कोपी का उपयोग करके कंकाल मांसपेशी तंतुओं में उपकोशिकीय ग्लाइकोजन वितरण का परिमाणीकरण

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy

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संचरण इलेक्ट्रॉन माइक्रोस्कोपी का उपयोग करके कंकाल मांसपेशी तंतुओं में उपकोशिकीय ग्लाइकोजन वितरण का परिमाणीकरण

3,685 Views

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08:32 min

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February 07, 2022

DOI:

10.3791/63347-v

DOI:

10.3791/63347-v

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08:32 min

February 07, 2022

•

2 Views

Rasmus Jensen, Niels Ørtenblad, Cristiano di Benedetto, Klaus Qvortrup, Joachim Nielsen

¹Research center for applied health science,University College South Denmark, ²Department of Sports Science and Clinical Biomechanics,University of Southern Denmark, ³Department of Biomedical Sciences, Core Facility for Integrated Microscopy,University of Copenhagen

Tags

Glycogen Distribution Subcellular Skeletal Muscle Fibers Transmission Electron Microscopy High Resolution Antibody-free Staining Potassium Ferrocyanide Glutaraldehyde Fixation Osmium Tetroxide Epoxy Resin Embedding Ultramicrotomy Semi-thin Sectioning Light Microscopy

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