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Quantification de la distribution subcellulaire du glycogène dans les fibres musculaires squelettiques à l’aide de la microscopie électronique à transmission
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Bioquímica
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JoVE Journal Bioquímica
Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy
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Quantification de la distribution subcellulaire du glycogène dans les fibres musculaires squelettiques à l’aide de la microscopie électronique à transmission

3,685 Views

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08:32 min

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February 07, 2022

DOI:

10.3791/63347-v

DOI:
10.3791/63347-v

•

08:32 min
February 07, 2022

•

2 Views
, , , ,
1Research center for applied health science,University College South Denmark, 2Department of Sports Science and Clinical Biomechanics,University of Southern Denmark, 3Department of Biomedical Sciences, Core Facility for Integrated Microscopy,University of Copenhagen

Tags

Glycogen DistributionSubcellularSkeletal Muscle FibersTransmission Electron MicroscopyHigh ResolutionAntibody-free StainingPotassium FerrocyanideGlutaraldehyde FixationOsmium TetroxideEpoxy Resin EmbeddingUltramicrotomySemi-thin SectioningLight Microscopy

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Jensen, R., Ørtenblad, N., di Benedetto, C., Qvortrup, K., Nielsen, J. Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy. J. Vis. Exp. (180), e63347, doi:10.3791/63347 (2022).

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