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Stereotaxic Surgical Approach to Microinject the Caudal Brainstem and Upper Cervical Spinal Cord via the Cisterna Magna in Mice
 

Stereotaxic Surgical Approach to Microinject the Caudal Brainstem and Upper Cervical Spinal Cord via the Cisterna Magna in Mice

Article DOI: 10.3791/63344-v • 07:07 min • January 21st, 2022
January 21st, 2022 •
1, 1, 1, 1
1Beth Israel Deaconess Medical Center, Harvard Medical School

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Stereotaxic surgery to target brain sites in mice commonly involves access through the skull bones and is guided by skull landmarks. Here we outline an alternative stereotaxic approach to target the caudal brainstem and upper cervical spinal cord via the cisterna magna that relies on direct visualization of brainstem landmarks.

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Stereotaxic Surgical Approach Microinjection Caudal Brainstem Upper Cervical Spinal Cord Cisterna Magna Mice Reproducible Route Of Access Precision Small Injection Volumes Restricted Regions Of Interest Animal Models Neurology Research VanderHorst Laboratory Oxygen Flow Nose Cone Stereotaxic Frame Ear Bars Lubricant On The Eyes Anteroflex The Mouse's Head Plastic Barrier Skull Reference Heating Pad Body Positioning Subcutaneous Injection
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