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分析编码人类α突触核蛋白的腺相关病毒载体诱导的帕金森病小鼠模型

JoVE Journal
Neurociência
Author Produced

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JoVE Journal Neurociência

Analyzing the Parkinson’s Disease Mouse Model Induced by Adeno-associated Viral Vectors Encoding Human α-Synuclein

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分析编码人类α突触核蛋白的腺相关病毒载体诱导的帕金森病小鼠模型

DOI:

10.3791/63313-v

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14:45 min

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July 29, 2022

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Daniela Elgueta, Ornella Chovar, Valentina Ugalde, Vanesa Sánchez-Guajardo, Alejandra Catenaccio, Felipe Court^3,4, Rodrigo Pacheco⁵

¹Laboratorio de Neuroinmunología,Fundación Ciencia & Vida, ²Center for Integrative Biology,Universidad Mayor, ³FONDAP Geroscience Center for Brain Health and Metabolism, ⁴Buck Institute for Research on Ageing, ⁵Facultad de Medicina y Ciencia,Universidad San Sebastián

Capítulos

00:13Introduction
02:25The Stereotaxic Surgery
05:38Determination of the Motor Performance Using the Beam Test
07:00Tissue Processing
08:51Representative Results
12:59Conclusion

Summary

Tadução automática

这项工作分析了在这种帕金森病临床前模型中诱导神经炎症，神经变性和运动障碍所需的载体剂量和暴露时间。这些编码人α突触核蛋白的载体被递送到黑质中，以概括与帕金森病相关的突触核蛋白病理学。

Tags

Keywords: Parkinson’s Disease Mouse Model Adeno-associated Viral Vectors Human α-synuclein Stereotaxic Delivery Nigrostriatal Pathway Neurodegeneration Neuroinflammation Motor Impairment Synuclein Pathology Microglial Activation T-cell Activation Aseptic Environment Analgesia Anesthesia

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