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Analyzing the Parkinson’s Disease Mouse Model Induced by Adeno-associated Viral Vectors Encoding Human α-Synuclein
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Neurosciences
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Journal JoVE Neurosciences
Analyzing the Parkinson’s Disease Mouse Model Induced by Adeno-associated Viral Vectors Encoding Human α-Synuclein

Analyzing the Parkinson’s Disease Mouse Model Induced by Adeno-associated Viral Vectors Encoding Human α-Synuclein

DOI:
10.3791/63313-v

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14:45 min

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July 29, 2022

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, , , , , ,
1Laboratorio de Neuroinmunología,Fundación Ciencia & Vida, 2Center for Integrative Biology,Universidad Mayor, 3FONDAP Geroscience Center for Brain Health and Metabolism, 4Buck Institute for Research on Ageing, 5Facultad de Medicina y Ciencia,Universidad San Sebastián

Chapitres

  • 00:13Introduction
  • 02:25The Stereotaxic Surgery
  • 05:38Determination of the Motor Performance Using the Beam Test
  • 07:00Tissue Processing
  • 08:51Representative Results
  • 12:59Conclusion

Summary

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This work analyzes the vector dose and exposure time required to induce neuroinflammation, neurodegeneration, and motor impairment in this preclinical model of Parkinson's disease. These vectors encoding the human α-synuclein are delivered into the substantia nigra to recapitulate the synuclein pathology associated with Parkinson's disease.

Tags

Keywords: Parkinson’s DiseaseMouse ModelAdeno-associated Viral VectorsHuman α-synucleinStereotaxic DeliveryNigrostriatal PathwayNeurodegenerationNeuroinflammationMotor ImpairmentSynuclein PathologyMicroglial ActivationT-cell ActivationAseptic EnvironmentAnalgesiaAnesthesia

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