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Analyzing the Parkinson’s Disease Mouse Model Induced by Adeno-associated Viral Vectors Encoding Human α-Synuclein
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Neurociência
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JoVE Journal Neurociência
Analyzing the Parkinson’s Disease Mouse Model Induced by Adeno-associated Viral Vectors Encoding Human α-Synuclein

Analyzing the Parkinson’s Disease Mouse Model Induced by Adeno-associated Viral Vectors Encoding Human α-Synuclein

5,348 Views

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14:45 min

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July 29, 2022

DOI:

10.3791/63313-v

DOI:
10.3791/63313-v

•

14:45 min
July 29, 2022

•

9 Views
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1Laboratorio de Neuroinmunología,Fundación Ciencia & Vida, 2Center for Integrative Biology,Universidad Mayor, 3FONDAP Geroscience Center for Brain Health and Metabolism, 4Buck Institute for Research on Ageing, 5Facultad de Medicina y Ciencia,Universidad San Sebastián

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This work analyzes the vector dose and exposure time required to induce neuroinflammation, neurodegeneration, and motor impairment in this preclinical model of Parkinson's disease. These vectors encoding the human α-synuclein are delivered into the substantia nigra to recapitulate the synuclein pathology associated with Parkinson's disease.

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Parkinson’s DiseaseMouse ModelAdeno-associated Viral VectorsHuman α-synucleinStereotaxic DeliveryNigrostriatal PathwayNeurodegenerationNeuroinflammationMotor ImpairmentSynuclein PathologyMicroglial ActivationT-cell ActivationAseptic EnvironmentAnalgesiaAnesthesia

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Elgueta, D., Chovar, O., Ugalde, V., Sánchez-Guajardo, V., Catenaccio, A., Court, F., Pacheco, R. Analyzing the Parkinson's Disease Mouse Model Induced by Adeno-associated Viral Vectors Encoding Human α-Synuclein. J. Vis. Exp. (185), e63313, doi:10.3791/63313 (2022).

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