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使用微图案弹性体对单细胞收缩力进行简化的高通量分析

JoVE Journal
Bioengenharia
Author Produced

This content is Free Access.

JoVE Journal Bioengenharia

Simplified, High-throughput Analysis of Single-cell Contractility using Micropatterned Elastomers

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使用微图案弹性体对单细胞收缩力进行简化的高通量分析

3,253 Views

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14:33 min

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April 08, 2022

DOI:

10.3791/63211-v

DOI:

10.3791/63211-v

•

14:33 min

April 08, 2022

•

1 Views

Lara Hairapetian¹, Enrico Cortes¹, Junyi Zhao¹, Yao Wang¹, Ricky Huang¹, Robert Damoiseaux^1,2, Ivan Pushkarsky¹

¹Forcyte Biotechnologies, ²University of California, Los Angeles

Summary

这项工作提出了一种灵活的方案，用于利用微孔格式的荧光标记弹性收缩表面（FLECS）技术，基于荧光蛋白微模式的可视化位移，对单细胞收缩力进行简化，无需干预的定量。

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