This content is Free Access.
Simplified, High-throughput Analysis of Single-cell Contractility using Micropatterned Elastomers
3,255 Views
•
14:33 min
•
April 08, 2022
DOI:
概要
Automatically generated
This work presents a flexible protocol for utilizing fluorescently labeled elastomeric contractible surfaces (FLECS) Technology in microwell format for simplified, hands-off quantification of single-cell contractile forces based on visualized displacements of fluorescent protein micropatterns.
Explore More Videos
Single-cell ContractilityMicropatterned ElastomersCell-generated Mechanical ForcesOrgan FunctionSmooth Muscle Cell ContractionContractile MechanismsCell Contractile FunctionDrug ScreeningMicrotechnologyFluorescently Labeled Elastomeric Contractable SurfacesProtein MicropatterningCell Position And ShapeCell Traction ForcesImage AnalysisCellular Force Biology
Read Article
Cite this Article
Hairapetian, L., Cortes, E., Zhao, J., Wang, Y., Huang, R., Damoiseaux, R., Pushkarsky, I. Simplified, High-throughput Analysis of Single-cell Contractility using Micropatterned Elastomers. J. Vis. Exp. (182), e63211, doi:10.3791/63211 (2022).
Copy