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Simplified, High-throughput Analysis of Single-cell Contractility using Micropatterned Elastomers
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Simplified, High-throughput Analysis of Single-cell Contractility using Micropatterned Elastomers
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April 08, 2022
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This work presents a flexible protocol for utilizing fluorescently labeled elastomeric contractible surfaces (FLECS) Technology in microwell format for simplified, hands-off quantification of single-cell contractile forces based on visualized displacements of fluorescent protein micropatterns.
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Single-cell ContractilityMicropatterned ElastomersCell-generated Mechanical ForcesOrgan FunctionSmooth Muscle Cell ContractionContractile MechanismsCell Contractile FunctionDrug ScreeningMicrotechnologyFluorescently Labeled Elastomeric Contractable SurfacesProtein MicropatterningCell Position And ShapeCell Traction ForcesImage AnalysisCellular Force Biology
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Hairapetian, L., Cortes, E., Zhao, J., Wang, Y., Huang, R., Damoiseaux, R., Pushkarsky, I. Simplified, High-throughput Analysis of Single-cell Contractility using Micropatterned Elastomers. J. Vis. Exp. (182), e63211, doi:10.3791/63211 (2022).
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