This work presents a flexible protocol for utilizing fluorescently labeled elastomeric contractible surfaces (FLECS) Technology in microwell format for simplified, hands-off quantification of single-cell contractile forces based on visualized displacements of fluorescent protein micropatterns.
Hairapetian, L., Cortes, E., Zhao, J., Wang, Y., Huang, R., Damoiseaux, R., Pushkarsky, I. Simplified, High-throughput Analysis of Single-cell Contractility using Micropatterned Elastomers. J. Vis. Exp. (182), e63211, doi:10.3791/63211 (2022).