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Simplified, High-throughput Analysis of Single-cell Contractility using Micropatterned Elastomers
JoVE Journal
Bioingegneria
Author Produced
This content is Free Access.
JoVE Journal Bioingegneria
Simplified, High-throughput Analysis of Single-cell Contractility using Micropatterned Elastomers

Simplified, High-throughput Analysis of Single-cell Contractility using Micropatterned Elastomers

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14:33 min

April 08, 2022

DOI:

14:33 min
April 08, 2022

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Summary

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This work presents a flexible protocol for utilizing fluorescently labeled elastomeric contractible surfaces (FLECS) Technology in microwell format for simplified, hands-off quantification of single-cell contractile forces based on visualized displacements of fluorescent protein micropatterns.

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