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מיצוי מולקולות גליקוגן בכבד לקביעת מבנה הגליקוגן
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04:50 min
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February 08, 2022
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1School of Chemistry and Molecular Biosciences,The University of Queensland, 2Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation,The University of Queensland, 3Jiangsu Provincial Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy,Xuzhou Medical University, 4Department of Pharmaceutical Analysis, School of Pharmacy,Xuzhou Medical University, 5Department of Bioinformatics, School of Medical Informatics and Engineering,Xuzhou Medical University, 6Joint International Research Laboratory of Agriculture and Agri-Product Safety, College of Agriculture,Yangzhou University, 7Glycation and Diabetes Group,Mater Research Institute-The University of Queensland, Translational Research Institute
Summary
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ריכוז סוכרוז אופטימלי נקבע למיצוי גליקוגן בכבד באמצעות צנטריפוגה הדרגתית של צפיפות סוכרוז. תוספת של שלב רתיחה של 10 דקות לעיכוב אנזימים מפרקים גליקוגן הוכחה כמועילה.
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Wang, Z., Liu, Q., Wang, L., Gilbert, R. G., Sullivan, M. A. The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination. J. Vis. Exp. (180), e63088, doi:10.3791/63088 (2022).
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