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استخراج جزيئات الجليكوجين في الكبد لتحديد بنية الجليكوجين
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04:50 min
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February 08, 2022
DOI:
1School of Chemistry and Molecular Biosciences,The University of Queensland, 2Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation,The University of Queensland, 3Jiangsu Provincial Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy,Xuzhou Medical University, 4Department of Pharmaceutical Analysis, School of Pharmacy,Xuzhou Medical University, 5Department of Bioinformatics, School of Medical Informatics and Engineering,Xuzhou Medical University, 6Joint International Research Laboratory of Agriculture and Agri-Product Safety, College of Agriculture,Yangzhou University, 7Glycation and Diabetes Group,Mater Research Institute-The University of Queensland, Translational Research Institute
Summary
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تم تحديد تركيز السكروز الأمثل لاستخراج الجليكوجين في الكبد باستخدام الطرد المركزي المتدرج لكثافة السكروز. أثبتت إضافة خطوة الغليان لمدة 10 دقائق لتثبيط إنزيمات تحلل الجليكوجين أنها مفيدة.
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Cite this Article
Wang, Z., Liu, Q., Wang, L., Gilbert, R. G., Sullivan, M. A. The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination. J. Vis. Exp. (180), e63088, doi:10.3791/63088 (2022).
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