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顺磁弛豫增强,用于检测和表征固有无序蛋白质的自缔合
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
DOI:

07:24 min

September 23, 2021

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Capítulos

  • 00:05Introduction
  • 00:49Conjugating the 3-Maleimido-PROXYL Nitroxide Spin Label
  • 01:38Preparing the NMR Sample for Measuring Intramolecular PRE
  • 02:11Setting Up the NMR Spectrometer and Experiment Specific Parameters
  • 03:51Setting Up the 1HNT2 Experiment
  • 04:58Making a Diamagnetic Sample by Reducing Spin-Label with Ascorbic Acid
  • 05:52Results: 1HNT2 Rates for Residues 171 – 264 of the Intrinsically Disordered Domain of EWSR1
  • 06:30Conclusion

Summary

Tadução automática

提出了一种应用顺磁弛豫增强核磁共振波谱来检测固有无序蛋白质中弱和瞬态分子间和分子内相互作用的方案。

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