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Stereotaksisk viral injektion og gradientindekslinseimplantation til dyb hjerne in vivo-calciumbilleddannelse
JoVE Journal
Neurociência
This content is Free Access.
JoVE Journal Neurociência
Stereotaxic Viral Injection and Gradient-Index Lens Implantation for Deep Brain In Vivo Calcium Imaging
DOI:

11:11 min

October 08, 2021

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Capítulos

  • 00:05Introduction
  • 01:09Stereotaxic Injection of Virus in the Medial Prefrontal Cortex (mPFC) of the Mouse Brain
  • 02:28Gradient Index (GRIN) Lens Implantation in the Medial Prefrontal Cortex (mPFC) of the Mouse Brain
  • 06:47Affixing Miniscope Holder (Base) to the Mouse Skull
  • 07:53Miniscope Mounting and In Vivo Ca2+ Imaging
  • 09:37Results: In Vivo Calcium Imaging Using a Miniscope
  • 10:32Conclusion

Summary

Tadução automática

Miniscope in vivo calcium imaging er en kraftfuld teknik til at studere neuronal dynamik og mikrokredsløb i frit opførende mus. Denne protokol beskriver udførelse af hjerneoperationer for at opnå god in vivo calciumbilleddannelse ved hjælp af et mikroskop.

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