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Stereotaxic Viral Injection and Gradient-Index Lens Implantation for Deep Brain In Vivo Calcium Imaging
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Neurowissenschaften
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JoVE Journal Neurowissenschaften
Stereotaxic Viral Injection and Gradient-Index Lens Implantation for Deep Brain In Vivo Calcium Imaging

Stereotaxic Viral Injection and Gradient-Index Lens Implantation for Deep Brain In Vivo Calcium Imaging

5,064 Views

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11:11 min

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October 08, 2021

DOI:

10.3791/63049-v

DOI:
10.3791/63049-v

•

11:11 min
October 08, 2021

•

9 Views
, , ,
1Department of Zoology and Physiology,University of Wyoming, 2School of Electrical Engineering & Computer Science,University of North Dakota, 3Department of Mathematics and Statistics,University of Wyoming

Summary

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Miniscope in vivo calcium imaging is a powerful technique to study neuronal dynamics and microcircuits in freely behaving mice. This protocol describes performing brain surgeries to achieve good in vivo calcium imaging using a miniscope.

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AI WritingDeep Brain ImagingIn Vivo Calcium ImagingMiniscopeStereotaxic Viral InjectionGRIN Lens ImplantationNeurowissenschaftenNeurobiologyNeural ActivityNeuron Recording

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Cite this Article

Thapa, R., Liang, B., Liu, R., Li, Y. Stereotaxic Viral Injection and Gradient-Index Lens Implantation for Deep Brain In Vivo Calcium Imaging. J. Vis. Exp. (176), e63049, doi:10.3791/63049 (2021).

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