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Stereotaxic Viral Injection and Gradient-Index Lens Implantation for Deep Brain In Vivo Calcium Imaging
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Neuroscience
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JoVE Journal Neuroscience
Stereotaxic Viral Injection and Gradient-Index Lens Implantation for Deep Brain In Vivo Calcium Imaging

Stereotaxic Viral Injection and Gradient-Index Lens Implantation for Deep Brain In Vivo Calcium Imaging

DOI:
10.3791/63049-v

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11:11 min

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October 08, 2021

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, , ,
1Department of Zoology and Physiology,University of Wyoming, 2School of Electrical Engineering & Computer Science,University of North Dakota, 3Department of Mathematics and Statistics,University of Wyoming

Chapters

  • 00:05Introduction
  • 01:09Stereotaxic Injection of Virus in the Medial Prefrontal Cortex (mPFC) of the Mouse Brain
  • 02:28Gradient Index (GRIN) Lens Implantation in the Medial Prefrontal Cortex (mPFC) of the Mouse Brain
  • 06:47Affixing Miniscope Holder (Base) to the Mouse Skull
  • 07:53Miniscope Mounting and In Vivo Ca2+ Imaging
  • 09:37Results: In Vivo Calcium Imaging Using a Miniscope
  • 10:32Conclusion

Summary

Automatic Translation

Automatic Translation

Miniscope in vivo calcium imaging is a powerful technique to study neuronal dynamics and microcircuits in freely behaving mice. This protocol describes performing brain surgeries to achieve good in vivo calcium imaging using a miniscope.

Tags

AI WritingDeep Brain ImagingIn Vivo Calcium ImagingMiniscopeStereotaxic Viral InjectionGRIN Lens ImplantationNeuroscienceNeurobiologyNeural ActivityNeuron Recording

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